LOCUS       OGA-pGEc2               7974 bp    DNA     circular     21-SEP-2016
FEATURES             Location/Qualifiers
     misc_feature    complement(7452..7581)
                     /note="3'-ITR"
     misc_feature    complement(7150..7364)
                     /note="BGH pA"
     rep_origin      1137..1804
                     /dnas_title="Col E1 origin"
                     /label=Col E1 origin
                     /vntifkey="33"
     CDS             129..989
                     /dnas_title="AmpR"
                     /label=AmpR
                     /vntifkey="4"
     misc_feature    1998..2127
                     /note="5'ITR"
     misc_feature    2208..2790
                     /note="CMV Promoter"
     misc_feature    2828..2960
                     /note="Artificial Intron"
     misc_feature    6602..7143
                     /note="WPRE"
     misc_recomb     complement(2974..3001)
                     /label=attB1
                     /note="attB1 site"
                     /note="recognized as attB1"
     misc_feature    5781..5803
                     /note="attB2"
     misc_feature    6581..6586
                     /note="BstB1 site"
     misc_feature    5804..6499
                     /note="Super GFP"
     misc_feature    6500..6550
                     /note="AviTag"
     misc_feature    6551..6574
                     /note="His Tag"
     PCR_frame       3011..5780
                     /note="Reading Frame"
                     /set="Set 1"
     misc_feature    3011..5758
                     /note="OPTIMIZED OGA coding region"
     misc_feature    5759..5780
                     /note="TEV"
     vector          join(5789..7974,1..2991)
                     /source="pGEc2-DEST"
                     /type="Destination Vector"
     clone           2992..5788
                     /source="OGA pDONR"
                     /type="Entry Clone"
     source          1..7974
                     /dnas_title="OGA-pGEc2"
ORIGIN      
        1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT
       61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA
      121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT
      181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT
      241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT
      301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG
      361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA
      421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA
      481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA
      541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA
      601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA
      661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA
      721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC
      781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC
      841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG
      901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA
      961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT
     1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA
     1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG
     1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA
     1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT
     1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC
     1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA
     1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA
     1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC
     1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA
     1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA
     1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG
     1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC
     1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG
     1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA
     1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA
     1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG
     1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG
     2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG
     2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT
     2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG
     2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT
     2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG
     2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT
     2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT
     2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG
     2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG
     2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC
     2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA
     2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
     2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC
     2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT
     2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA
     2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC
     3001 TCGCGCCACC ATGGTCCAGA AAGAATCTCA GGCCACCCTG GAAGAGAGAG AGTCCGAGCT
     3061 GTCTAGCAAT CCTGCTGCCT CTGCTGGCGC TTCTCTGGAA CCTCCAGCTG CTCCAGCTCC
     3121 TGGCGAAGAT AATCCTGCAG GCGCTGGCGG AGCTGCTGTG GCTGGTGCTG CTGGGGGAGC
     3181 TAGAAGATTT CTGTGTGGCG TGGTGGAAGG CTTCTACGGC AGACCTTGGG TCATGGAACA
     3241 GCGGAAAGAG CTGTTCAGAC GGCTGCAGAA GTGGGAGCTG AACACCTACC TGTACGCCCC
     3301 CAAGGACGAC TACAAGCACA GAATGTTCTG GCGGGAAATG TACAGCGTGG AAGAGGCCGA
     3361 ACAGCTGATG ACACTGATCA GCGCCGCCAG AGAGTACGAG ATCGAGTTCA TCTATGCCAT
     3421 CAGCCCTGGC CTGGACATCA CCTTCAGCAA CCCTAAAGAG GTGTCCACCC TGAAGCGGAA
     3481 GCTGGATCAG GTGTCCCAGT TCGGCTGTAG AAGCTTCGCC CTGCTGTTCG ACGACATCGA
     3541 CCACAACATG TGTGCCGCCG ACAAAGAAGT GTTCTCCAGC TTTGCCCACG CCCAGGTCAG
     3601 CATCACCAAC GAGATCTACC AGTATCTGGG CGAGCCCGAG ACATTCCTGT TCTGCCCTAC
     3661 AGAGTACTGC GGCACCTTCT GCTACCCCAA TGTGTCTCAG AGCCCCTACC TGAGAACAGT
     3721 GGGAGAGAAG CTGCTGCCAG GCATCGAAGT GCTGTGGACA GGACCTAAGG TGGTGTCTAA
     3781 AGAGATCCCC GTCGAGAGCA TCGAGGAAGT GTCCAAGATC ATCAAGAGGG CCCCTGTGAT
     3841 CTGGGACAAC ATCCACGCCA ACGACTACGA CCAGAAGAGA CTGTTCCTGG GCCCCTACAA
     3901 GGGAAGAAGC ACCGAGCTGA TTCCCAGACT GAAGGGCGTG CTGACCAACC CCAATTGCGA
     3961 GTTCGAGGCC AACTACGTGG CCATCCATAC CCTGGCCACC TGGTACAAGA GCAACATGAA
     4021 CGGCGTGCGG AAGGACGTCG TGATGACCGA TAGCGAGGAT AGCACCGTGT CCATCCAGAT
     4081 CAAGCTGGAA AACGAGGGCA GCGACGAGGA CATCGAGACA GATGTGCTGT ACTCCCCCCA
     4141 GATGGCCCTG AAACTGGCTC TGACAGAGTG GCTGCAGGAA TTCGGAGTCC CTCACCAGTA
     4201 CAGCTCCAGA CAGGTGGCAC ATTCTGGCGC CAAAGCCTCT GTGGTGGATG GAACACCTCT
     4261 GGTGGCTGCC CCTTCTCTGA ATGCCACAAC CGTGGTCACC ACCGTGTATC AGGAACCCAT
     4321 CATGTCTCAG GGCGCTGCTC TGAGCGGAGA GCCTACCACA CTGACCAAAG AGGAAGAGAA
     4381 GAAACAGCCC GATGAGGAAC CTATGGATAT GGTGGTCGAG AAGCAGGAAG AAACCGATCA
     4441 CAAGAACGAC AACCAGATCC TGAGCGAGAT CGTGGAAGCC AAGATGGCCG AGGAACTGAA
     4501 GCCCATGGAC ACAGACAAAG AGTCTATCGC CGAGAGCAAG AGCCCCGAGA TGAGCATGCA
     4561 GGAGGACTGC ATCTCCGATA TCGCCCCCAT GCAGACCGAC GAGCAGACCA ACAAAGAACA
     4621 GTTCGTGCCA GGCCCCAATG AGAAGCCACT GTATACCGCC GAACCCGTGA CACTGGAAGA
     4681 TCTGCAGCTG CTGGCCGACC TGTTTTACCT GCCTTATGAG CACGGACCAA AGGGCGCTCA
     4741 GATGCTGAGA GAATTCCAGT GGCTGAGGGC CAACTCTAGC GTGGTGTCCG TGAACTGCAA
     4801 GGGCAAGGAT TCCGAGAAGA TCGAAGAGTG GCGGTCCAGA GCCGCCAAGT TCGAAGAAAT
     4861 GTGTGGCCTC GTGATGGGCA TGTTCACCAG ACTGTCCAAC TGCGCCAACC GGACCATCCT
     4921 GTACGACATG TACTCCTACG TGTGGGACAT CAAGTCCATT ATGTCTATGG TCAAGAGCTT
     4981 CGTGCAGTGG CTCGGATGCA GAAGCCATTC CTCCGCCCAG TTTCTGATCG GCGACCAGGA
     5041 ACCTTGGGCC TTTAGAGGTG GACTGGCCGG CGAATTTCAG AGACTGCTCC CTATCGACGG
     5101 CGCCAATGAT CTGTTCTTCC AGCCTCCTCC ACTGACCCCC ACCAGCAAGG TGTACACAAT
     5161 CAGACCCTAC TTCCCTAAGG ACGAGGCCTC CGTGTACAAG ATCTGTAGAG AGATGTACGA
     5221 CGACGGCGTG GGACTGCCTT TCCAGTCTCA GCCTGACCTG ATCGGAGACA AACTCGTGGG
     5281 AGGGCTGCTG AGCCTGTCCC TGGATTACTG CTTTGTGCTC GAGGATGAGG ATGGCATCTG
     5341 TGGCTATGCC CTGGGCACAG TGGATGTGAC CCCCTTTATC AAGAAGTGCA AGATCTCCTG
     5401 GATCCCTTTT ATGCAGGAAA AGTACACCAA GCCCAACGGC GATAAGGAAC TGTCCGAGGC
     5461 CGAGAAAATC ATGCTGAGCT TTCACGAGGA ACAGGAGGTG CTGCCTGAAA CCTTCCTGGC
     5521 CAATTTCCCC AGCCTGATTA AGATGGACAT CCACAAGAAA GTGACCGACC CCAGCGTGGC
     5581 CAAGAGCATG ATGGCTTGTC TGCTCAGTAG CCTGAAGGCC AATGGCTCCA GAGGCGCCTT
     5641 TTGTGAAGTG CGGCCCGACG ACAAGCGGAT TCTCGAGTTT TACAGCAAGC TGGGCTGCTT
     5701 CGAGATCGCC AAAATGGAAG GGTTCCCCAA AGACGTGGTC ATCCTGGGCA GATCCCTCga
     5761 aaacttgtac tttcaaggct CCAGCTTTCT TGTACAAAGT GGTATGAGCA AGGGCGAGGA
     5821 ACTGTTCACC GGCGTGGTGC CCATCCTGGT GGAACTGGAT GGCGACGTGA ACGGCCACAA
     5881 GTTCTCTGTG CGGGGAGAGG GCGAAGGCGA CGCCACAAAT GGCAAGCTGA CCCTGAAGTT
     5941 CATCTGCACC ACCGGCAAGC TGCCCGTGCC TTGGCCTACC CTCGTGACCA CACTGACCTA
     6001 CGGCGTGCAG TGCTTCAGCA GATACCCCGA CCATATGAAG CGGCACGACT TCTTCAAGAG
     6061 CGCCATGCCC GAGGGCTACG TGCAGGAACG GACCATCAGC TTCAAGGACG ACGGCACCTA
     6121 CAAGACCAGA GCCGAAGTGA AGTTCGAGGG CGACACCCTC GTGAACCGGA TCGAGCTGAA
     6181 GGGCATCGAT TTCAAAGAGG ACGGCAACAT CCTGGGCCAC AAGCTGGAGT ACAACTTCAA
     6241 CAGCCACAAC GTGTACATCA CCGCCGACAA GCAGAAGAAC GGCATCAAGG CCAACTTCAA
     6301 GATCCGGCAC AACGTGGAAG ATGGCAGCGT GCAGCTGGCC GACCACTACC AGCAGAACAC
     6361 CCCCATCGGA GATGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA CCCAGAGCGT
     6421 GCTGAGCAAG GACCCCAACG AGAAGCGGGA CCACATGGTG CTGCTGGAAT TCGTGACCGC
     6481 CGCTGGCATC ACCCACGGCA TGAGCGGCCT GAACGACATC TTCGAGGCCC AGAAAATCGA
     6541 GTGGCACGAG CACCACCATC ATCACCATCA CCACTGATGA TTCGAAAAAT AATAAGGATC
     6601 CAATCAACCT CTGGATTACA AAATTTGTGA AAGATTGACT GGTATTCTTA ACTATGTTGC
     6661 TCCTTTTACG CTATGTGGAT ACGCTGCTTT AATGCCTTTG TATCATGCTA TTGCTTCCCG
     6721 TATGGCTTTC ATTTTCTCCT CCTTGTATAA ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT
     6781 GTGGCCCGTT GTCAGGCAAC GTGGCGTGGT GTGCACTGTG TTTGCTGACG CAACCCCCAC
     6841 TGGTTGGGGC ATTGCCACCA CCTGTCAGCT CCTTTCCGGG ACTTTCGCTT TCCCCCTCCC
     6901 TATTGCCACG GCGGAACTCA TCGCCGCCTG CCTTGCCCGC TGCTGGACAG GGGCTCGGCT
     6961 GTTGGGCACT GACAATTCCG TGGTGTTGTC GGGGAAGCTG ACGTCCTTTC CATGGCTGCT
     7021 CGCCTGTGTT GCCACCTGGA TTCTGCGCGG GACGTCCTTC TGCTACGTCC CTTCGGCCCT
     7081 CAATCCAGCG GACCTTCCTT CCCGCGGCCT GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT
     7141 TCGAGATCTG CCTCGACTGT GCCTTCTAGT TGCCAGCCAT CTGTTGTTTG CCCCTCCCCC
     7201 GTGCCTTCCT TGACCCTGGA AGGTGCCACT CCCACTGTCC TTTCCTAATA AAATGAGGAA
     7261 ATTGCATCGC ATTGTCTGAG TAGGTGTCAT TCTATTCTGG GGGGTGGGGT GGGGCAGGAC
     7321 AGCAAGGGGG AGGATTGGGA AGACAATAGC AGGCATGCTG GGGACTCGAG TTAAGGGCGA
     7381 ATTGCCGATT AGGATCTTCC TAGAGCATGG CTACGTAGAT AAGTAGCATG GCGGGTTAAT
     7441 CATTAACTAC AAGGAACCCC TAGTGATGGA GTTGGCCACT CCCTCTCTGC GCGCTCGCTC
     7501 GCTCACTGAG GCCGGGCGAC CAAAGGTCGC CCGACGCCCG GGCTTTGCCC GGGCGGCCTC
     7561 AGTGAGCGAG CGAGCGCGCA GCCTTAATTA AGAGCTCATG GCGCGCCTAG GCCTTGACGG
     7621 CCTTCCGCCA ATTCGCCCTA TAGTGAGTCG TATTACGTCG CGCTCACTGG CCGTCGTTTT
     7681 ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT AATCGCCTTG CAGCACATCC
     7741 CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA GGCCCGCACC GAAACGCCCT TCCCAACAGT
     7801 TGCGCAGCCT GAATGGCGAA TGGGAGCGCC CTGTAGCGGC CACTCAACCC TATCTCGGTC
     7861 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG
     7921 ATTTAACAAA AATTTAACGC GAATTTTAAC AAAATATTAA CGCTTACAAT TTAG
//

